The aim of this study was to examine molecular alterations on the protein level in lesions of
oral lichen planus (OLP), oral squamous cell carcinoma (OSCC) and healthy mucosa. Global
protein profiling methods based on liquid chromatography coupled to mass spectrometry were
used, with a special emphasis on evaluation of deregulated extracellular matrix molecules
expression, as well as on analyses of insulin-like growtg factor 2 (IG2F) and insulin-like
growth factor 2 receptor (IGFR2) expression in healthy mucosa, OLP and OSCC tissues by
comparative semiquantitative immunohistochemistry.
Mass spectrometry based proteomics profiling of healthy mucosa, OLP and OSCC tissues (and
accompanied histologically unaltered tissues, respectively) identified 55 extracellular
matrix proteins. Twenty among identified proteins were common to all groups of samples.
Statistically significant difference between final IGF2 and IGF2R IRS scores in favour to
IGF2R may further corroborate the IG2FR antitumor role in OLP and OSCC where it acts as a
negative regulator of IGF2 activity.