Purpose: to study the effect of estrogen replacement on muscle hypertrophy in response to 12
weeks resistance training in postmenopausal women Hypothesis: Transdermal administration of
17-β estradiol enhance the anabolic effect of resistance training on muscle mass, strength in
postmenopausal women Primary outcome: Muscle hypertrophy (muscle CSA, muscle fiber type
specific CSA) Secondary outcome: Messenger RiboNucleic Acid (mRNA)(real-time PCR), protein
(western blotting), satellite cell proliferation and activation, Strength, functional test,
muscle protein synthese.
Design: Two groups of healthy postmenopausal women (½-5 years after menopause) who have not
performed regular resistance exercise (<1 times per week) the last two years. Randomized,
controlled intervention study +/- transdermal administration of estrogen (17-β estradiol).
Both groups perform 12 weeks of supervised progressive resistance training (3/week) aiming to
induce muscle hypertrophy in the legs.