Atopic dermatitis and psoriasis are two skin diseases often associated with bacterial
infections and inflammation. Studies indicate that skin cells from these patients may have
some changes that make these patients more susceptible to bacterial infections. Inflammatory
environment may have an effect on the function of skin cells.
The purpose of this study is to learn more about skin cells (keratinocytes and fibroblasts)
and how they regulate skin barrier function. To study this we need to establish skin cells
that can be grown in the laboratory. We will use small skin biopsies from patients with
atopic dermatitis, psoriasis and healthy people as a source of these cells. Since these skin
cells have a limited lifetime when grown in laboratory as part of the project we would like
to modify them, which allows them to grow for long time in the research laboratory. Some of
the collected skin biopsies and isolated skin cells will be used to examine what gene
products they make.