The first aim of the study is to obtain from chirurgical micro biopsy myoblast in culture
which will differentiate in Diaphragm fiber. Then these fibers will be submitted under
mechanical stress condition similar to those imposed in vivo by mechanical ventilation to
validate in human a model in vitro of diaphragm dysfunction induced by mechanical
ventilation. Then the second part of the study will be to evaluate with this model, the
efficiency of an antioxidant therapy.