The aim of the present research protocol was to evaluate the effect of probiotics daily used,
on the plaque acidogenicity of children. The null hypothesis was that the plaque
acidogenicity would remain unchanged and not associate with the use of probiotics. Moreover,
the in situ study will be carried out to investigate the plaque formation as biofilm in
demineralised enamel specimens following the regular consumption of a probiotic product
compared to a control group. Metabolic of the biofilm formed on of the enamel specimens
resulting from the consumption will be evaluated over a period of 8 weeks.