Rationale: Proteins released from muscle during and shortly after exercise, often referred to
as myokines, may be central to our understanding of the cross-talk during and after exercise
between skeletal muscles and other organs, in particular the liver. So far only a few
myokines are identified (e.g. IL-6, IL-8, IL-15, TNF-alpha). Taking into account the role of
these several known myokines in developing insulin resistance, revealing new putative
myokines might provide valuable information and a direction for future research on the
pathogenesis and treatment of type 2 diabetes mellitus.
Objective: The objective of the present study is to identify novel myokines, expression of
which is altered in skeletal muscle after a single bout of exercise.
Study design: experimental study. Study population: Ten healthy, male subjects between 40 and
60 years of age and BMI < 30 kg/m2, will participate in this study.
Intervention: A single exercise bout that consists of one hour one-legged cycling on a
adapted recumbent cycle ergometer at a submaximal rate. The non-exercising leg will serve as
control for the exercising leg.
Main study outcomes: Main study outcomes include upregulation of genes in skeletal muscle
after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels
of selected proteins after exercise.